Protein and Antibody Display and Screening

Connect antibodies with targets through phage display

Phage display links displayed proteins or peptides to their encoding sequences. Antibody binding, enrichment, and sequencing identify candidate antigens or epitopes for antibody profiling and target validation.

Phage library representing porcine kidney-associated proteins

AlloDx has a phage display library representing porcine kidney-associated proteins for antibody-recognition studies in xenotransplantation. Projects can encompass sample incubation, target enrichment, and sequencing analysis. Protein and peptide coverage, library version, and compatibility are confirmed per project.

Baseline and follow-up samples can be compared for antigen-recognition profiles, differential epitopes, and associated pathways. Screening signals nominate candidates but do not alone demonstrate pathogenicity or establish transplant rejection.

Link screening with validation

1. Confirm research goals and library coverage
2. Plan antibody binding, controls, and replicates
3. Sequence enriched material and analyze candidate epitopes
4. Validate with ELISA, Luminex, or functional assays

Understand the limits of display-based screening

PhIP-seq depends on the antigen fragments represented in its library. Linear peptide libraries have limited coverage of conformational epitopes and post-translational modifications. Negative findings do not exclude relevant antibodies, and positive findings require independent validation.

RNA display, MIPSA, and protein microarrays are related but distinct approaches. This service focuses on phage screening; other platforms are considered according to target properties and project requirements.

Technical background and references

• Mohan et al. · PhIP-Seq characterization of serum antibodies (2018)
• Lin & Chen · Bacterial proteome microarray technology in biomedical research